Branched oligonucleotides
WebJan 30, 2024 · FIGS. 15A-15C show branched oligonucleotides of the invention, FIG. 15A —formed by annealing three oligonucleotides. The longer linking oligonucleotides may comprise a cleavable region in the form of unmodified RNA, DNA or UNA; FIG. 15B —asymmetrical branched oligonucleotides with 3′ and 5′ linkages to the linkers or … WebThe unique architecture of branched oligonucleotides mimicking lariat RNA introns [Wallace and Edmons, Proc. Natl. Acad. Sci. USA 80, 950-954 (1983)] was exploited to study compounds that associate as two parallel duplexes with intercalating C/C+ base pairs (i-motif DNA) [Gehring et al. Nature 363, …
Branched oligonucleotides
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WebSep 8, 2003 · Branched oligonucleotides (b-oligonucleotides) based on a novel branching monomer were used for site-specific sequence alteration in vivo. With a stable integrated mutated enhanced green ... Web[47,48] Beside this modification, branched oligonucleotides [49] and dumbbell-shaped circular RNAs [50][51][52] have been developed with excellent resistance to 3'-exonucleases. Recently, a ...
WebJun 9, 2006 · During the synthesis of oligonucleotides by the standard phosphoramidite method using 2′-deoxycytidine- derivatized solid support, a side reaction was observed that gave rise to the formation of high molecular weight N-branched oligomers having two identical chains linked to the 3′-terminal 2′-deoxycytidine.Postsynthesis treatment with … WebOct 26, 2007 · The branched ODN was successfully synthesized using 5-[3,5-bis(trifluoromethyl)phenyl]-1H-tetrazole and (2R,8aS)-( … Synthesis of branched oligonucleotides with three different sequences using …
WebDec 8, 2024 · In the phosphoramidite method for synthesizing oligonucleotides, the chain elongation (phosphorylation) is carried out by activating phosphoramidite building blocks using acidic activators, such as 1 H -tetrazole (p K a = 4.8) on polymer supports [ 8 ]. The p K a value of activators used in DNA/RNA synthesis varies from 4.5 to 7.0. WebJan 16, 2024 · The synthesis of branched oligonucleotides and the results of the assembly of nanostructures will be published later. MATERIALS AND METHODS. The constructed models of NA nanostructures consist of RNA and DNA duplexes and linkers. Duplexes are represented by rigid cylinders; their sizes were determined using duplex …
WebThe captured viral RNA target is then hybridized to branched oligonucleotides (hence the name ‘branched’ DNA assay), which in turn are hybridized to enzyme-conjugated oligonucleotides that can be quantified as above. Performance characteristics of most commercially available assays are similar. Most assays have a lower limit of ...
WebMay 29, 2010 · Beside that, the branched side chain of nucleoside 1 containing two terminal triple bonds allows ‘double’ functionalization with two reporter groups per residue instead of ‘mono’ functionalization as it is the case for the earlier described compound 2.In this context, high density functionalization of oligonucleotides is of current interest. 29, 31, 38, 39, … diamond collector钻石收集加强版WebAbstract. A solid-phase synthesis for phosphate-branched oligonucleotides is described. The method is based on coupling of a single nucleoside phosphorodiamidite to terminal hydroxyl functions of two solid-supported oligonucleotides. After oxidation of the phosphite triester obtained to a phosphate triester, the third branch is assembled by ... diamond collector gameWebApr 11, 1995 · All the oligonucleotides prepared contained two T11 sequences, and the branched and looped oligomers an additional alternating CT sequence. The melting experiments revealed that the branched oligonucleotides form relatively weak hybrid (double/triple helix) complexes with the single-stranded oligodeoxyribonucleotide, … diamond collective san bernardinoWebApr 11, 1995 · All the oligonucleotides prepared contained two T 11 sequences, and the branched and looped oligomers an additional alternating CT sequence. The melting experiments revealed that the branched oligonucleotides form relatively weak hybrid (double/triple helix) complexes with the single-stranded oligodeoxyribonucleotide, … circuit breaker singaporeWebApr 15, 2012 · Branched oligonucleotides IX and X were synthesized using an appropriately protected pentaerythritol linker . The stability of triplexes formed with these oligonucleotides was studied by thermal denaturation in Tris–HCl buffer solution (15 mM, pH 7.0) containing 25 mM NaCl and 5 mM MgCl 2 ( Figs. 3 and S2 ). circuit breaker singleWebOligonucleotides Bio-Synthesis offers one of the largest selection of modifications for DNA and RNA synthesis. We provide branched DNA (bDNA) synthesis using 5-Me-dC Brancher to produce a multimer comb-like constructs 1 which can be use to amplify the … diamond collectorWebOct 8, 2024 · Nucleoside phosphoramidite derivatives containing two protected primary hydroxyl functions have been incorporated into synthetic oligonucleotides as 'branching monomers'. With selective deprotection, multiple identical copies of an additional oligonucleotide can be incorporated to form fork- or comb-like structures for use as signal ... diamond collector nrich