2024 Bulked segregant analysis bsa dna sequencing

Bulked segregant analysis bsa dna sequencing

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August undefined, 2024

WebBulk segregation analysis (BSA) utilizes a strategy of pooling individuals with extreme phenotypes to conduct economical and rapidly linked marker screening or quantitative … WebMar 6, 2024 · Bulked segregant analysis (BSA), coupled with next-generation sequencing, allows the rapid identification of both qualitative and quantitative trait loci (QTL), and this technique is referred to as BSA-Seq here.

Bulked segregant analysis using next-generation …

WebApr 14, 2024 · Bulked segregant analysis sequencing (BSA-seq) may be used to find the allele frequency (AF) of single-nucleotide polymorphisms by generating a DNA pool of offspring with extreme... WebSequencing this stretch of DNA identified the central portion as the putative Mat1-2 locus of M graminicola. ... NGS-based mapping approaches using bulked segregant analysis … installing fiber optic cable underground

Bulked-Segregant Analysis Coupled to Whole Genome …

Web2 days ago · The enormous amount of short reads generated by the new DNA sequencing technologies call for the development of fast and accurate read alignment programs. ... WebJan 20, 2024 · The BSA-NGS analysis revealed that two candidate loci from 1.7 to 2.0 Mbp and 5.6 to 6.2 Mbp on chromosome (Chr) 9 were associated with dwarfism. We validated these SNPs in F2 progeny using a high-resolution melting assay. Based on the results, we identified a perfect cosegregation region of 1,872,994 to 1,866,655 bp on Chr 9. WebFeb 4, 2024 · Next-generation sequencing-based bulked segregant analysis without sequencing the parental genomes Genomic regions that control traits of interest can be rapidly identified using BSA-Seq, a technology in which next-generation sequencing is applied to bulked segregant analysis (BSA). jiffy lube leduc alberta

Combined use of specific length amplified fragment …

Bulk segregation analysis in the NGS era: a review of its teenage …

WebBulked-segregant analysis is a strategy of genetic mapping by constructing two bulks of individuals with contrasting phenotypes from the segregating population. Bulked-segregant analysis sequencing (BSA-seq), based on next-generation sequencing technology, is an effective tool in mapping genetic loci underlying agronomic traits (especially for ... WebJan 1, 2024 · Combining next-generation sequencing (NGS) and bulked-segregant analysis (BSA) [, ... About 5 μg of DNA from each bulk was used for the next generation … jiffy lube leavenworth kansasWebNov 25, 2024 · Therefore, this study aimed to identify significant QTLs associated with NCLB by utilizing next-generation sequencing-based bulked-segregant analysis (BSA). Parental lines GML71 (resistant) and Gui A10341 (susceptible) were used to develop segregating population F 2. Two bulks with 30 plants each were further selected from the … installing files from packages wavs

"WebTwo bulks with 30 plants each were further selected from the segregating population for sequencing along with the parental lines. High throughput sequencing data was used for BSA. We identified 10 QTLs on Chr 1, Chr 2, Chr 3, … " - Bulked segregant analysis bsa dna sequencing

Bulked segregant analysis bsa dna sequencing

Bulked segregant analysis using next-generation …

WebMay 31, 2024 · Next-generation sequencing (NGS)-based bulked-segregant analysis (BSA) approaches have been proven successful for rapidly mapping genes in plant … WebMay 21, 2024 · Bulked segregant analysis (BSA) was first applied in plant genetics by Michelmore et al. . All alleles must be present when DNA is bulked from a group of …

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WebMay 7, 2012 · Bulked segregant analysis (BSA) is a method used to rapidly identify genetic markers linked to a genomic region associated with the selected phenotype . … WebApr 13, 2024 · Here, bulked segregant RNA-Seq was used to identify two candidate genes for melanoid, which, in comparison to the wild type, is characterized by increased …

WebBulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked … WebNov 6, 2024 · Here, we describe a bulked-segregant analysis sequencing (BSA-Seq) protocol for cloning mutant genes in maize. Our simple strategy can be used to quickly …

WebNov 3, 2011 · We describe a statistical framework for QTL mapping using bulk segregant analysis (BSA) based on high throughput, short-read sequencing. Our proposed … WebIn this study, the Cry2Ab resistance gene in T. ni was mapped to Chromosome 17 by genetic linkage analyses using a whole genome resequencing approach, and was then finely mapped using RNA-seq-based bulked segregant analysis (BSA) and amplicon sequencing (AmpSeq)-based fine linkage mapping to a locus containing two genes, …

WebApr 14, 2024 · Bulked segregant RNA-seq (BSR-seq) couples BSA with RNA-seq (Liu et al., 2012) and can be used not only to identify differentially expressed genes (DEGs) but …

WebDNA extraction, library construction, and sequencing. Allele frequency estimates in BSA analysis depend on the variation in segregant samples and sequencing technology. … jiffy lube lake worth flWebApr 14, 2024 · Bulked segregant RNA-seq (BSR-seq) couples BSA with RNA-seq (Liu et al., 2012) and can be used not only to identify differentially expressed genes (DEGs) but also single-nucleotide polymorphisms (SNPs) that are tightly linked to targeted traits (Du et al., 2024). BSR-seq has been successfully applied in analyses of many species. installing fighting butt on fly rodWebMar 9, 2024 · 从f2群体中选择黄绿叶植株和绿叶植株构建极端表型的dna 混池，采用bsa分析发现，在第3号染色体短臂和长臂上各存在1个明显的Δsnp指数曲线的峰（图3），这两个区间可能存在控制gy157s 黄绿叶表型的候选基因。 jiffy lube knoxville tnWebJul 6, 2024 · BSA（bulked segregant analysis）又叫集群分离分析法，该方法主要是选择双亲群体分离后代中具有极端表型的个体进行混样，通过比较不同极端混样池之间的多态性标记的差异，从而筛选出与性状相关的分子标记，实现目标基因的定位。 BSA 分析早期使用的都是传统分子标记，根据标记与性状共分离（紧密连锁）得到定位区间，不过由于其标 … installing field drainage tileWebDec 12, 2024 · Bulked segregant analysis (BSA), which is the fastest and most accurate way to spot molecular markers connected to our trait of interests, while associated with high-throughput tools, such as next … jiffy lube live backstreet boysWebJan 28, 2024 · Key message Using bulked segregant analysis of exome sequence, we fine-mapped the ABA-hypersensitive mutant ERA8 in a wheat backcross population to … installing fiona on windowsWebApr 11, 2024 · A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype. ... followed by a bulked segregant analysis (BSA) using whole genome … jiffy lube lexington ky locations