WebApr 23, 2015 · of Cre recombinase during development (Schmidt-Supprian and Rajewsky, 2007). In addition, we obtained Vglut2-ires-Cre and Chat-ires-Cre mice (Rossi et al., 2011; Vong et al., 2011), which provided important tools for global control of sensory neurons and motor neurons respectively. Calcium imaging experiments in acute WebFind answers to questions regarding benefits, claims, prescriptions and more. FAQ. Our contact information is specific to state. If you are a member, log in for personalized …
Characterization of social behavior in young and middle …
WebDec 20, 2024 · A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. The neo cassette was excised with Flp recombinase. Cre recombinase activity is reported in all cholinergic neurons. This allele … WebJul 2, 2024 · To visualize potential cholinergic neurons in the cortex, we genetically labeled all Chat-expressing cells with tdTomato (Chat ires … the most dangerous person ever
Generation of a whole-brain atlas for the cholinergic …
WebNov 3, 2015 · Adult male ChAT-IRES-cre 57, Vglut2-IRES-cre 58 and Vgat-IRES-Cre 59 mice and non-cre-expressing littermate mice (8–12 weeks, 20–25 g; n=86 in vivo and n=24 in vitro) were used in this study. Web006410 B6;129S6- Chat tm2(cre)Lowl /J ChAT-IRES-Cre knock-in mice express Cre recombinase in cholinergic neurons, without disrupting endogenous Chat expression. … WebWe found that most GFP-positive neurons in Chat- ires-Cre:Ai47 mice (89-98% in cortical and subcortical brain re- gions) also expressed ChAT, indicating that the genetic labeling used in this ... the most dangerous nerf gun