WebDec 19, 2024 · FUCCI-HeLa cells were synchronized at the G1/S boundary by using double thymidine block (DTB) as described previously . In detail, cells were treated with 2.5 mM thymidine for 16 h and were released back with normal medium for 8 h. After second thymidine treatment with 2.5 mM thymidine for an additional 16 h, cells were arrested … Web2 Centre for Diabetes, Chronic Diseases and Ageing, School of Science and Technology, Nottingham Trent University, Nottingham, UK. 3 The John van Geest Cancer Research Centre, School of Science and Technology, Nottingham Trent University, Nottingham, UK. [email protected]. PMID: 34085219. DOI: 10.1007/978-1-0716-1538-6_9.
An evaluation of the double thymidine block for …
WebSep 1, 2024 · The double thymidine block is a highly effective and widely used protocol for the synchronization of cells to the early S phase (see Note 1). Excess thymidine inhibits … WebSep 5, 2024 · The protocol to synchronize cells at G1/S boundary by using double thymidine block is presented, which could help to obtain cells at distinct cell cycle stage through synchronization. Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct … spider-man unlimited marvel wiki
DNA replication fork speed underlies cell fate changes and
WebSep 11, 2024 · We approached this question with the real-time transcription reporter lines, by synchronizing cultures to the G1/S phase boundary using a double thymidine block and imaging continuously for 24 h ... WebChen G, Deng X (2024) Cell synchronization by double thymidine block. Bio Protoc 8:e2994 Sonoda E (2006) Synchronization of cells. Subcell Biochem 40:415–418 Samaké S, Smith LC (1996) Synchronization of cell division in eight-cell bovine embryos produced in vitro: effects of nocodazole. Mol Reprod Dev 44:486–492 WebAdherent HeLa cells were synchronized using a double thymidine block method. Thymidine (Sigma) was added to the cell culture media at a final concentration of 2 mM from a 100 mM stock solution in water. The cells were incubated for 16 h at 37 °C, after which the culture media was removed, the cells were washed three times with sterile … spider man\u0027s height