WebAnnexin V FITC/Propidium Iodide Staining Solution Prepare sufficient Annexin V FITC/Propidium Iodide Staining Solution to stain up to 100 samples by adding 10 µl of Annexin V FITC Reagent (Item No. 601281) and 2.5 µl of Cell-Based Propidium Iodide Solution (Item No. 10011234) to 5 ml of 1X Binding Buffer. Mix well. WebMar 10, 2016 · The FITC labeling reaction operates on deprotonated amino groups. At pH 3, the amino groups will be almost entirely protonated, so the reaction will not go well. Adjust the pH to a basic pH of at ...
Fluorescein isothiocyanate - Wikipedia
Web19002 - AT - GFP/FITC Longpass. Add some data! Use the legend to add fluorochromes, filters sets and individual filters to the plot. All transmission and blocking (OD) data are actual, measured spectra of representative production lots. Spectra varies slightly from lot to lot. Optical density values in excess of 6 may appear noisy because such ... WebFeb 10, 2024 · Py 3-FITC can be used to detect TGF-β1-induced collagen type I. HFL-1 cells were treated with TGF-β1 for 0 h (a–c) or 48 h (d–f) and then stained with Py 3-FITC (a,d) and collagen type I antibody (b,e), and their merged images are shown (c,f). Collagen type I expression was increased by 48 h of TGF-β1 treatment. open age hammersmith and fulham
Fluorescein isothiocyanate (FITC) AAT Bioquest
WebWe offer a broad range of fluorescein products including protein and antibody labeling kits, primary and secondary antibody conjugates, bioconjugates, and more. Learn more here: … WebFITC - FRRL Industrial Trading Corporation Oil and Gas Makati City, NCR 239 followers An industrial trading company serving Oil & Gas (Upstream & Downstream), Power … WebThe FITC solution is then diluted (1:50, 1.5:100, 1:100, 1:200) with PBS buffer by the students. 2.2. Bioconjugation. The protocol used for the modification of BSA with FITC is reported in literature 1 and is very simple: 1) Students are asked to prepare a 2 mg/ml BSA solution in 0.1 M sodium carbonate, pH 9. open a ghost kitchen brand