WebSpecifically, every day cells were detached from a subset of wells in the multi-well plates by incubation with 0.25% trypsin-EDTA solution (Sigma-Aldrich, St. Louis, MO, USA) for 10 min. Detached cells were then stained with trypan blue stain (0.4% in PBS) (Lonza, Walkersville, MD, USA) for 2 min at room temperature and counted using a hemocytometer. WebCompany profile page for Sigma-Aldrich Chemie Holding GmbH including stock price, company news, press releases, executives, board members, and contact information
Sigma-Aldrich Chemie Holding GmbH - Company Profile and News
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