WebRNA fragments were dephosphorylated using T4 PNK (New England Biolabs, M0201S), and ligated to pre-adenylated 3′-universal miRNA cloning linker (New England Biolabs, S1315S) by T4 RNA ligase 2, truncated (New England Biolabs, M0242S) at … WebApr 3, 2024 · The T4 DNA ligase was detected with a linear range of 4.8 × 10-3 to 6.0 U mL-1, achieving a detection limit of 1.6 × 10-3 U mL-1. The outcome indicated that the developed nanozyme might be extended to a broad range of practical applications. ... which only needs mixing of two broad commercialized reagents at room temperature, to harvest gold ...
Efficient DNA ligation by selective heating of DNA ligase with a …
WebFeb 1, 2024 · 10× T4 DNA ligase buffer and 400 U/μL T4 DNA Ligase. 10. 20 mg/mL bovine serum albumin. 11. 20 mg/mL Proteinase K. 12. 5 M sodium chloride. 13. ... Mix at room temperature for 10 min (see Note 1). 5. Add 2.5 M glycine solution to a final concentration of 0.2 M (see Note 2). 6. Webblunt-end DNA ligation in only 5 minutes at room temperature (see Fig.1).The kit includes T4 DNA Ligase and a specially formulated 5X Rapid Ligation Buffer optimized for fast and efficient DNA ligation. The efficiency of the fast ligation is analogous to that obtained with T4 DNA Ligase in a standard 1 hour ligation. The ligation reaction egyptian trading system
T4 DNA Ligase - Thermo Fisher Scientific
WebSep 12, 2016 · The PCR product was then treated with T4 ligase-T4 polynucleotide kinase-DpnI before transformation into XL10-GOLD ultracompetent cells. The resulting pCMV-T7-HH-EV71-HDV infectious clone was subjected to DNA sequencing. ... For nuclear visualization, cells were treated with 0.01% 4’,6-diamidino-2-phenylindole (DAPI, Sigma) … WebBenefits: • Fast 15-minute incubation at room temperature • Phosphorylaton can be performed with DNA or oligonucleotides in water, TE, or 1X Anza buffers The Anza T4 PNK Kit is part of the Anza™ Restriction Enzyme Cloning … WebAllow the microtube to slowly cool to room temperature (<60 min). Thermocycler. ... Heat to 95 °C and maintain the temperature for 2 min. Cool to 25 °C over 45 min. ... This buffer is typically used with T4 DNA Ligase. 50 mM Tris-HCl, pH … fold money into triangle